Cyclin D1 overexpression is associated with poor prognosis in oropharyngeal cancer
© Lin et al.; licensee BioMed Central Ltd. 2013
Received: 3 December 2012
Accepted: 11 March 2013
Published: 19 March 2013
To determine the biological characteristics of oropharyngeal squamous cell carcinoma (OpSCC) and related outcome.
Patients (N=60) with primary OpSCC from 2000 to 2005 were retrospectively identified from Pathology database and the outcome was confirmed through chart review. Among these, 41 biopsy samples with enough tissues were retrieved to construct a tissue microarray for detection of the presence of high-risk human papillomavirus (HPV) using Chromogenic in situ hybridization (CISH) as well as the expression of p16 and cyclin D1 using immunohistochemistry.
Main outcome measures
Among 60 patients, 39 (65%) patients had no recurrence or died without disease at the last follow-up (disease-free survival or Group 1), and 21 (35%) patients had persistent disease or died of disease (progression-free survival or Group 2). Although follow-up time was twice as long in group 1 (4.7 ± 2.2 vs. 2.0 ± 1.6 years; P < 0.0001), there was no difference between the 2 groups in age, gender, smoking/alcohol habits, TNM staging and treatment modalities. Among those 41 cases with available tumour tissues, there was no difference in HPV status and p16 expression between the 2 groups but a significant difference in cyclin D1 expression (P = 0.05). Using Kaplan-Meir survival analysis and log-rank test, cyclin D1 overexpression was highly associated with a poor prognosis when comparing time to outcome (P < 0.0001).
Cyclin D1 overexpression is a potential prognostic marker of OpSCC.
KeywordsOropharyngeal squamous cell carcinoma (OpSCC) Human papillomavirus (HPV) p16 Cyclin D1 Disease free survival Prognosis
The incidence of oropharyngeal squamous cell carcinoma (OpSCC), particularly those arising in the base of the tongue and in the tonsillar region, rises every year between 1973 and 2004 . This increasing trend occurs despite the decrease in the incidence of oral cavity, laryngeal and hypopharyngeal cancers as well as the decreased prevalence in smoking, which is a primary risk factor for these cancers . Molecular studies have identified human papillomavirus (HPV) as a causative agent in 60% to 80% of patients with OpSCC . HPV-16 accounts for a large majority of HPV-positive OpSCC (80-90%) compared to other oncogenic types such as HPV-18, 31 or 33 [4, 5].
HPV-positive OpSCCs have distinct risk factors and molecular profiles. These tumours are thought to be associated with certain types of sexual behavior, but not to tobacco smoking or alcohol use. On a molecular level, almost all HPV-positive tumours express wild-type p53 tumour suppressor genes. Expression of HPV viral E6 and E7 oncoproteins inactivates p53 and the retinoblastoma protein pRb. p16 protein expression is subsequently elevated due to loss of the pRb negative feedback loop . p16 then inhibits cyclinD1-CDK4/6 (cyclin-dependent kinase 4/6) complexes, which are important regulators for the cell cycle to progress from the G1 phase to the S phase. As a result, HPV-positive cancers are thought to be associated with downregulation of cyclin D1 expression [7, 8]. In other head and neck cancers that are not typically related to HPV, cyclin D1 overexpression has been linked with poor outcome, particularly in hypopharyngeal cancers . However there is not a large amount of evidence suggesting its prognostic significance in oropharyngeal cancer.
Given the worldwide growing epidemic of HPV-associated oropharyngeal cancers, characterization of HPV-associated molecular markers is becoming increasingly important for determining their impact on the prognosis of disease and for guiding the development of new therapeutic interventions. The objective of our study was to determine the association of cyclin D1 and HPV status in OpSCC and its relevance on patient outcome. Since cyclin D1 overexpression has been associated with a poor prognosis in cancers of other head and neck regions, we hypothesize it is associated with a poor prognosis in oropharyngeal cancer.
Patient and tumour tissue
This is a retrospective case series. The study has obtained approval from the Research Ethics Board at the University of British Columbia and has been in compliance with research conduct guidelines at this institution. A total of 60 patients (n = 60) with primary OpSCC from the year 2000 to 2005 were retrospectively identified from the Vancouver General Hospital Pathology database. This duration was chosen so that all patients have had at least 5 years of follow-up after their initial treatment. These patients were confirmed to have no previous history of cancer and their outcome information were collected through electronic chart review from the patient information database at the British Columbia Cancer Agency (BCCA). Demographic and clinical data were also collected from chart review, including age, gender, smoking status, alcohol intake, treatment received, follow-up time and survival status. Tumour staging using the TNM classification  was determined by review of clinical notes, radiology reports, surgical notes and pathology reports. Among the included patients, 41 (68%) formalin-fixed biopsy specimens with enough tissues were retrieved from the pathology archive for the construction of tissue microarrays.
Tissue microarray (TMA) construction
Tissue microarray was constructed as previously described . The most representative areas of tumours were chosen by the pathologist (CFP). Tissue cores of 0.6 mm in diameter from these representative areas of formalin-fixed pffin-embedded (FFPE) tumour blocks were taken by a manual tissue arrayer (Beecher Instrument, SI, USA). The cores were then transferred in duplicates to the recipient TMA block. The recipient blocks were then serially cut into 4 μm tissue sections for chromogenic in situ hybridization and immunohistochemistry.
Chromogenic in situ Hybridization (CISH) for HPV high-risk subtypes and HPV-16/18
High-risk HPV subtypes and specifically HPV subtypes 16 as well as 18 in FFPE TMA sections were detected by using chromogenic in situ hybridization (CISH). Two HPV probes were examined in this study: HPV-HR (cocktail for high-risk types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68, DAKO, Denmark), and HPV-16/18 (DAKO, Denmark). In brief, each of the tissue sections were depffinized, rehydrated and pretreated with target retrieval solution (DAKO) for 20–40 minutes at 97°C. After antigen retrieval with Proteinase K (Sigma-Aldrich, Canada) and blocking, the slides were hybridized with a biotinylated HPV-HR probe and HPV-16/18-specific probe. After initial binding of streptavidin horseradish peroxidase complex to the probe, signal amplification was performed using biotinyl tyramide. Positive hybridization signals were visualized by adding the chromogenic substrate diaminobenzidine (DAB). SiHa and SCC-9 cell lines were used as a positive and negative control, respectively. Slides were scored positive for HPV if a nuclear, punctuate pattern of signals was observed in > 60% of the tumour nuclei, which was indicative of integrated HPV DNA into the host genome. The scoring was performed by the same pathologist (CFP).
Immunohistochemistry (IHC) for p16 and cyclin D1
Antibodies used in immunohistochemistry for p16 and cyclin D1
Ready to use
Room temperature, 30 min
Nuclear and cytoplasmic
CINtec® Histology Kit (Roche mtm laboratories AG, Heidelberg, Germany)
Cyclin D1 (92G2) Rabbit mAb #2978 (Cell Signaling, Ontario, CA)
All statistical analyses were performed using SPSS/PASW Statistics (version 19, SPSS Inc., Chicago, IL). The Fisher’s exact test was used to compare dichotomous (e.g., gender, alcohol consumption and N staging) or other categorical (e.g., smoking status, T staging, TNM staging, and treatment) variables. Student’s t-test was used to compare pmetric variable (e.g., age and follow-up time). Disease-free survival was estimated using the Kaplan-Meier method with log-rank test for determining statistical significance. Disease-free survival was defined as time from the day of cancer diagnosis to the date of death from OpSCC or to the date of last follow-up. Multivariate analysis was not performed given the small sample size of the study. A P value of 0.05 or less was considered statistically significant.
Comparison of patient characteristics between two outcome groups
Total (N = 60)
Group 1a(N=39, 65%)
Group 2a(N = 21, 35%)
Mean age at diagnosis (yr ± SD)
60.4 ± 11.7
59.8 ± 11.3
61.4 ± 12.6
Former smokers (quit > 5 yr)
Never or light
TNM staging c
Surgery (S/T) only
Radiation (XRT) onlyd
Combined S/T and XRT
Mean Follow-Up Time (yr ± SD)
4 ± 2.4
4.7 ± 2.2
2 ± 1.6
HPV status and p16 expression
Comparison of tumour HPV DNA, p16 and cyclin D1 status between outcome groups 1 and 2
Group 1 (N=39, 65%)
Group 2 (N=21, 35%)
Cyclin D1 expression and disease-free survival
Twelve tumours were cyclin D1-positive (12/41, 29%) and twenty-nine tumours were cyclin D1-negative (29/41, 71%). Among the cyclin D1-negative tumours, 21 (78%) were positive for p16, a currently accepted surrogate marker for the presence of HPV DNA. For those tumours that were cyclin D1-positive (n = 14), only 43% (n = 6) were positive for p16. Using Fisher’s exact test, there was no difference in cyclin D1 expression between the two outcome groups (Table 3). However, the improved disease-free survival over time was significant in the cyclin D1-negative group using the log-rank analysis (P < 0.0001, hazard ratio 18.0, 95% confidence interval 4.37 to 74.03; Figure 2D).
Cyclin D1 overexpression has been reported in squamous cell carcinoma in a variety of head and neck subsites including the larynx, hypopharynx and tongue [9, 14, 15]. Its prognostic significance in OpSCC has been contradictory [16–22]. Our study has demonstrated cyclin D1 overexpression is associated with a poor prognosis in primary OpSCC. Cyclin D1 is a well-known oncogene in a variety of human cancers. It is a key regulator of cell proliferation. It promotes cell cycle progression through G1 phase by forming complexes with CDK4 and CDK6, which in turn phosphorylate the retinoblastoma protein pRb . This causes pRb to release the E2F transcription factor, which then activates genes essential for cell cycle progression from G1 phase to S phase, during which cells proliferate . It has been suggested that cyclin D1 plays a role in the late phase of tumour progression given its correlation with lymph node metastases, histological aggressiveness and poor prognosis .
Cyclin D1 overexpression was higher in patients with adverse outcomes in our study, but the difference was not statistically significant. With the log-rank analysis, however, the difference in disease-free survival over time was statistically significant between the cyclin D1-positive and cyclin D1-negative groups. Although this study had a small sample size, we fitted a multivariate Cox Proportional Hazard model to examine the potential joint and interaction effects among markers (data not shown). The analysis showed that cyclin D1 overexpression was the only and independent variable demonstrating significance in predicting poor outcome in disease-free survival. Despite the small number of patients in our study, our finding strongly suggest that cyclin D1 could potentially be a useful prognostic marker for OpSCC.
In our study, HPV DNA was detected in 56% of OpSCC with the entire 56% being p16-positive. This is consistent with the reported numbers from previous studies [25, 26]. 9.8% of tumours were p16-positive and HPV-negative, which has also been reported in the literature . Overall, our study supports p16 as an effective surrogate marker for high-risk HPV infection with high sensitivity and specificity (85% and 100%, respectively). Cyclin D1 expression should be downregulated in HPV-positive head and neck squamous cell carcinoma as a result of pRb suppression [7, 24]. In our study, 78% (N=21) of cyclin D1-negative tumours were associated with p16 overexpression. This inverse relationship is consistent with the hypothesis that HPV positivity and subsequent p16 upregulation leads to suppression of cyclin D1 expression.
Our study has not found any significant association between smoking or alcohol intake and tumour HPV status. Interestingly, all the nonsmokers showed p16/HPV positivity but 62.5% of smokers have also shown p16/HPV positivity. This may argue against the current literature observation that HPV-positive tumours tend to occur in non-smokers and non-drinkers .
One of the limitations of this study is that it was a retrospective review, thus it relied on the accuracy of written or dictated medical records. A second limitation is the small sample size. We were only able to identify a total number of 60 cases of primary oropharyngeal carcinoma with known outcome from 2000 to 2005. Thus we may not have enough patients in order to detect a statistical significance in the expression of markers between the two outcome groups. We were also not able to perform a multivariate analysis due to the small sample size. The next step of this project is to widen our search through the BC Cancer Registry in order to identify more primary oropharyngeal cancer cases and to retrieve their tumour tissues for tissue microarray construction. A prospective arm of the study is also underway to recruit patients with new oropharyngeal carcinoma diagnosis to participate in our investigation.
Our preliminary results have demonstrated cyclin D1 overexpression is a potential prognostic marker of primary oropharyngeal squamous cell carcinoma. Further understanding of these genetic alterations leading to malignant progression is critical to future secondary prevention strategies and therapeutic interventions for patients with HPV-positive oropharyngeal squamous cell carcinoma.
Written informed consent was obtained from patients who participated in this study for publication of this report and any accompanying images.
TL is supported by a scholarship of the Anandamahidol Foundation and CFP is supported by the Scholar Award of the Michael Smith Foundation for Health Research.
- Chaturvedi AK, Engels EA, Anderson WF, Gillison ML: Incidence trends for human papillomavirus–related and –unrelated oral squamous cell carcinomas in the United States. J Clin Oncol. 2008, 26: 612-9. 10.1200/JCO.2007.14.1713.PubMedView ArticleGoogle Scholar
- Sturgis EM, Ang KK: The epidemic of HPV-associated oropharyngeal cancer is here: is It time to change Our treatment pdigms?. J Natl Compr Canc Netw. 2011, 9: 665-73.PubMedGoogle Scholar
- Chaturvedi AK, Engels EA, Pfeiffer RM: Human papillomavirus and rising oropharyngeal cancer incidence in the united states. J Clin Oncol. 2011, 29: 4294-301. 10.1200/JCO.2011.36.4596.PubMedPubMed CentralView ArticleGoogle Scholar
- Kreimer AR, Clifford GM, Boyle P, Franceschi S: Human Papillomavirus Types in Head and Neck Squamous Cell Carcinomas Worldwide: A Systematic Review. Cancer Epidemiol Biomarkers Prev. 2005, 14: 467-75. 10.1158/1055-9965.EPI-04-0551.PubMedView ArticleGoogle Scholar
- Herrero R, Castellsagué X, Pawlita M: Human papillomavirus and oral cancer: the international agency for research on cancer multicenter study. J Natl Cancer Inst. 2003, 95: 1772-83. 10.1093/jnci/djg107.PubMedView ArticleGoogle Scholar
- Adelstein DJ, Ridge JA, Gillison ML: Head and neck squamous cell cancer and the human papillomavirus: Summary of a National Cancer Institute State of the Science Meeting, November 9–10, 2008. 2009, Washington, D.C: Head & Neck, 1393-422. 31Google Scholar
- Andl T, Kahn T, Pfuhl A: Etiological involvement of oncogenic human papillomavirus in tonsillar squamous cell carcinomas lacking retinoblastoma cell cycle control. Cancer Res. 1998, 58: 5-12.PubMedGoogle Scholar
- Li W, Thompson CH, Cossart YE: The expression of key cell cycle markers and presence of human papillomavirus in squamous cell carcinoma of the tonsil. Head Neck. 2004, 26: 1-9. 10.1002/hed.10335.PubMedView ArticleGoogle Scholar
- Masuda M, Hirakawa N, Nakashima T, Kuratomi Y, Komiyama S: Cyclin D1 overexpression in primary hypopharyngeal carcinomas. Cancer. 1996, 78: 390-5. 10.1002/(SICI)1097-0142(19960801)78:3<390::AID-CNCR2>3.0.CO;2-O.PubMedView ArticleGoogle Scholar
- Edge SBBD, Compton CC: AJCC Cancer Staging Manual. 2010, New York, NY: Springer, 7Google Scholar
- Chen B, van den Brekel MWM, Buschers W, Balm AJM, van Velthuysen M-LF: Validation of tissue array technology in head and neck squamous cell carcinoma. Head Neck. 2003, 25: 922-30. 10.1002/hed.10308.PubMedView ArticleGoogle Scholar
- O’Regan EM, Toner ME, Finn SP: p16INK4A genetic and epigenetic profiles differ in relation to age and site in head and neck squamous cell carcinomas. Hum Pathol. 2008, 39: 452-8. 10.1016/j.humpath.2007.08.004.PubMedView ArticleGoogle Scholar
- Wang J, Wang X, Gong W, Mi B, Liu S, Jiang B: Increased expression of β-catenin, phosphorylated glycogen synthase kinase 3β, cyclin D1, and c-myc in laterally spreading colorectal tumors. J Histochem Cytochem. 2009, 57: 363-71.PubMedPubMed CentralView ArticleGoogle Scholar
- Mineta H, Miura K, Takebayashi S: Cyclin D1 overexpression correlates with poor prognosis in patients with tongue squamous cell carcinoma. Oral Oncol. 2000, 36: 194-8. 10.1016/S1368-8375(99)00078-0.PubMedView ArticleGoogle Scholar
- Jares P, Fernández PL, Campo E: PRAD-1/cyclin D1 gene amplification correlates with messenger RNA overexpression and tumor progression in human laryngeal carcinomas. Cancer Res. 1994, 54: 4813-7.PubMedGoogle Scholar
- Perisanidis C, Perisanidis B, Wrba F: Evaluation of immunohistochemical expression of p53, p21, p27, cyclin D1, and Ki67 in oral and oropharyngeal squamous cell carcinoma. J Oral Pathol Med. 2012, 41: 40-6. 10.1111/j.1600-0714.2011.01071.x.PubMedView ArticleGoogle Scholar
- Smith B, SGLCD: MOlecular marker expression in oral and oropharyngeal squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2001, 127: 780-5.PubMedGoogle Scholar
- Michalides R, vVNJKPP: OVerexpression of cyclin d1 indicates a poor prognosis in squamous cell carcinoma of the head and neck. Arch Otolaryngol Head Neck Surg. 1997, 123: 497-502. 10.1001/archotol.1997.01900050045005.PubMedView ArticleGoogle Scholar
- Rasamny JJ, Allak A, Krook KA: Cyclin D1 and FADD as Biomarkers in Head and Neck Squamous Cell Carcinoma. Otolaryngology. Head Neck Surg. 2012, 146: 923-31. 10.1177/0194599811435052.View ArticleGoogle Scholar
- Hong AM, Dobbins TA, Lee CS: Use of cyclin D1 in conjunction with human papillomavirus status to predict outcome in oropharyngeal cancer. Int J Cancer. 2011, 128: 1532-45. 10.1002/ijc.25479.PubMedView ArticleGoogle Scholar
- Yu Z, Weinberger PM, Haffty BG: Cyclin D1 is a valuable prognostic marker in oropharyngeal squamous cell carcinoma. Clin Cancer Res. 2005, 11: 1160-6.PubMedGoogle Scholar
- Capaccio P, Pruneri G, Carboni N: Cyclin D1 expression is predictive of occult metastases in head and neck cancer patients with clinically negative cervical lymph nodes. Head Neck. 2000, 22: 234-40. 10.1002/(SICI)1097-0347(200005)22:3<234::AID-HED5>3.0.CO;2-3.PubMedView ArticleGoogle Scholar
- Baldin V, Lukas J, Marcote MJ, Pagano M, Draetta G: Cyclin D1 is a nuclear protein required for cell cycle progression in G1. Genes Dev. 1993, 7: 812-21. 10.1101/gad.7.5.812.PubMedView ArticleGoogle Scholar
- Li Y, Nichols MA, Shay JW, Xiong Y: Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb. Cancer Res. 1994, 54: 6078-82.PubMedGoogle Scholar
- Rischin D, Young RJ, Fisher R: Prognostic significance of p16INK4A and human papillomavirus in patients with oropharyngeal cancer treated on TROG 02.02 Phase III trial. J Clin Oncol. 2010, 28: 4142-8. 10.1200/JCO.2010.29.2904.PubMedPubMed CentralView ArticleGoogle Scholar
- Fakhry C, Westra WH, Li S: Improved survival of patients with human papillomavirus–positive head and neck squamous cell carcinoma in a prospective clinical trial. J Natl Cancer Inst. 2008, 100: 261-9. 10.1093/jnci/djn011.PubMedView ArticleGoogle Scholar
- Ang KK, Harris J, Wheeler R: Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med. 2010, 363: 24-35. 10.1056/NEJMoa0912217.PubMedPubMed CentralView ArticleGoogle Scholar
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