| Primer | Forward (5′ → 3′) | Reverse(5′ → 3′) |
|---|
| VP1 | AGTCCTCCCACGTCACTCAC | TGATCACTAGGGTGGCATCA |
| IFN-α | GCCTCGCCCTTTGCTTTACT | CTGTGGGTCTCAGGGAGATCA |
| RIG-I | CTGGACCCTACCTACATCCTG | GGCATCCAAAAAGCCACGG |
| MAVS | CAGGCCGAGCCTATCATCTG | GGGCTTTGAGCTAGTTGGCA |
| NLRP3 | CGTGAGTCCCATTAAGATGGAGT | CCCGACAGTGGATATAGAACAGA |
| TLR1 | TGAACCTCAAGCACTTGGACC | CCCATAAGTCTCTCCTAAGACCA |
| TLR2 | ATCCTCCAATCAGGCTTCTCT | GGACAGGTCAAGGCTTTTTACA |
| TLR3 | TTGCCTTGTATCTACTTTTGGGG | TCAACACTGTTATGTTTGTGGGT |
| TLR4 | AGACCTGTCCCTGAACCCTAT | CGATGGACTTCTAAACCAGCCA |
| TLR5 | TCCCTGAACTCACGAGTCTTT | GGTTGTCAAGTCCGTAAAATGC |
| TLR7 | TCGTGGACTGCACAGACAAG | GGTATGTGGTTAATGGTGAGGGT |
| TNF-α | CCTCTCTCTAATCAGCCCTCTG | GAGGACCTGGGAGTAGATGAG |
| IL-6 | ACTCACCTCTTCAGAACGAATTG | CCATCTTTGGAAGGTTCAGGTTG |
| IL-1β | ATGATGGCTTATTACAGTGGCAA | GTCGGAGATTCGTAGCTGGA |
| NF-κB | AACAGAGAGGATTTCGTTTCCG | TTTGACCTGAGGGTAAGACTTCT |
| IL-7 | TTCCTCCCCTGATCCTTGTTC | CTTGCGAGCAGCACGGAATA |
| IL-10 | GACTTTAAGGGTTACCTGGGTTG | TCACATGCGCCTTGATGTCTG |
| IL-12A | CCTTGCACTTCTGAAGAGATTGA | ACAGGGCCATCATAAAAGAGGT |
| IL-17A | AGATTACTACAACCGATCCACCT | GGGGACAGAGTTCATGTGGTA |
| IL-18 | TCTTCATTGACCAAGGAAATCGG | TCCGGGGTGCATTATCTCTAC |
| COX2 | GAATCATTCACCAGGCAAATT | TCTGTACTGCGGGTGGAACA |
| EP1 | ACCTTCTTTGGCGGCTCTC | CCAACACCAGCATTGGGCT |
| EP2 | GCTCCTTGCCTTTCACGATTT | AGGATGGCAAAGACCCAAGG |
| EP3 | AGCTTATGGGGATCATGTGC | TTTCTGCTTCTCCGTGTGTG |
| EP4 | CGCTCGTGGTGCGAGTATT | AGGGGTCTAGGATGGGGTTC |
| GAPDH | CGCTCTCTGCTCCTCCTGTT | CATGGGTGGAATCATATTGG |
