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Fig. 1 | Journal of Otolaryngology - Head & Neck Surgery

Fig. 1

From: Introduction and expression of PIK3CAE545K in a papillary thyroid cancer BRAFV600E cell line leads to a dedifferentiated aggressive phenotype

Fig. 1

Expression of activated PIK3CAE545K mutation into a BRAF-mutant PTC cell line leads to an increase in cell proliferation. A Immunoblot analysis of the LAM1 cell line containing the BRAFV600E-mutant with an empty vector (LAM1EV) and the LAM1 cell line with the PIK3CAE545K added (LAM1:PIK3CAE545K). Cells were collected and whole cell lysates were prepared with 20 µg total protein loaded per well. α-tubulin was used as a loading control. B Brightfield microscopy images were collected 24 h after plating of the LAM1EV cell line and the LAM1:PIK3CAE545K cell lines. Cells were seeded at a density of 150,000 cells per well. C Cells were seeded at a density of 2400 cells per well and incubated for 24 h. Cellular proliferation was measured with the addition of PrestoBlue to cells, which were then incubated for one hour prior to plate readings. Relative Fluorescent Units (RFU) on the y-axis represent the fluorescence of the PrestoBlue output. Changes in cellular proliferation were measured at baseline, 24, 48 and 72 h for both LAM1EV and LAM1:PIK3CAE545K cell lines

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