Fig. 4

Introduction of PIK3CA^E545K led to loss of well-differentiated thyroid cancer markers in vivo. A LAM1^EV and LAM1:PIK3CA^E545K were implanted in the hind flanks of nude mice. Mice were sacrificed at 14 weeks post-injection and tumours were dissected from flanks and stored in formalin for FFPE preparation and IHC. B Dissected tumours were weighed (mg) for each specimen and compared between the LAM1^EV and LAM1:PIK3CA^E545K groups. Individual tumour volumes of dissected specimens were calculated using the formula: [length x (width)²] × 0.52 and compared between cell line groups. (C,D) Tumours were collected, stained (C), and quantified (D) to determine relative differences in protein expression of thyroid differentiation markers thyroid transcription factor-1 (TTF-1), PAX8, thyroglobulin and B-catenin between the LAM1^EV and LAM1:PIK3CA^E545K models