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Table 2 Limma statistics and fold change

From: Serum microRNA profiling to distinguish papillary thyroid cancer from benign thyroid masses

MiRNA p-value adj. p.Value t-Test Mean target Ct Mean calibrator Ct ddCT Fold change
Benign vs. Controla       
 hsa-miR-150-5p <0.001 <0.001 6.2 30.35 27.79 2.6 −5.9
 hsa-miR-342-3p <0.001 <0.01 4.8 32.34 30.51 1.8 −3.5
 hsa-let-7b-5p <0.001 0.01 −4.0 29.92 31.36 −1.4 2.7
 hsa-miR-191-5p 0.001 0.04 −3.5 31.73 32.76 −1.0 2.0
PTC vs. Controlb        
 hsa-let-7b-5p <0.001 <0.001 −5.6 29.40 31.36 −2.0 3.9
 hsa-miR-150-5p <0.001 <0.001 5.7 30.11 27.79 2.3 −5.0
 hsa-miR-146a-5p <0.001 0.02 3.7 33.49 32.21 1.3 −2.4
 hsa-miR-342-3p <0.001 0.03 3.7 31.88 30.51 1.4 −2.6
 hsa-miR-191-5p <0.001 0.03 −3.5 31.75 32.76 −1.0 2.0
 hsa-miR-93-5p <0.001 0.03 −3.5 28.68 29.53 −0.85 1.8
PTC vs. Benignc        
 hsa-miR-10a-5p <0.01 0.5 −3.1 35.45 35.95 −0.50 1.4
 hsa-miR-146a-5p 0.01 0.5 2.7 33.49 32.93 0.56 −1.5
 hsa-miR-199b-3p 0.01 0.5 2.7 33.33 32.79 0.54 −1.5
 hsa-let-7b-5p 0.02 0.5 −2.4 29.40 29.92 −0.51 1.4
  1. Fold-changes and significance of changes by t-Test/Limma. ddCT = delta delta CT – measure of relative expression quantification (comparative cycle threshold)
  2. Adjusted p-value accounts for multiple comparisons
  3. aFalse discovery rate (FDR) <5 %
  4. bFDR <1 %
  5. cFDR <50 %