Serum microRNA profiling to distinguish papillary thyroid cancer from benign thyroid masses

Table 2 Limma statistics and fold change

MiRNA	p-value	adj. p.Value	t-Test	Mean target Ct	Mean calibrator Ct	ddCT	Fold change
Benign vs. Control^a
hsa-miR-150-5p	<0.001	<0.001	6.2	30.35	27.79	2.6	−5.9
hsa-miR-342-3p	<0.001	<0.01	4.8	32.34	30.51	1.8	−3.5
hsa-let-7b-5p	<0.001	0.01	−4.0	29.92	31.36	−1.4	2.7
hsa-miR-191-5p	0.001	0.04	−3.5	31.73	32.76	−1.0	2.0
PTC vs. Control^b
hsa-let-7b-5p	<0.001	<0.001	−5.6	29.40	31.36	−2.0	3.9
hsa-miR-150-5p	<0.001	<0.001	5.7	30.11	27.79	2.3	−5.0
hsa-miR-146a-5p	<0.001	0.02	3.7	33.49	32.21	1.3	−2.4
hsa-miR-342-3p	<0.001	0.03	3.7	31.88	30.51	1.4	−2.6
hsa-miR-191-5p	<0.001	0.03	−3.5	31.75	32.76	−1.0	2.0
hsa-miR-93-5p	<0.001	0.03	−3.5	28.68	29.53	−0.85	1.8
PTC vs. Benign^c
hsa-miR-10a-5p	<0.01	0.5	−3.1	35.45	35.95	−0.50	1.4
hsa-miR-146a-5p	0.01	0.5	2.7	33.49	32.93	0.56	−1.5
hsa-miR-199b-3p	0.01	0.5	2.7	33.33	32.79	0.54	−1.5
hsa-let-7b-5p	0.02	0.5	−2.4	29.40	29.92	−0.51	1.4

Fold-changes and significance of changes by t-Test/Limma. ddCT = delta delta CT – measure of relative expression quantification (comparative cycle threshold)
Adjusted p-value accounts for multiple comparisons
^aFalse discovery rate (FDR) <5 %
^bFDR <1 %
^cFDR <50 %

ISSN: 1916-0216