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Fig. 2 | Journal of Otolaryngology - Head & Neck Surgery

Fig. 2

From: In vivo Wnt pathway inhibition of human squamous cell carcinoma growth and metastasis in the chick chorioallantoic model

Fig. 2

Analysis of ALDH positive cells in UM-SCC-1 CAM xenografts. a Flow cytometry of unstained sample of UM-SCC-1 primary tumor cells grown in the CAM assay. b Flow cytometry of UM-SCC-1 CAM xenograft tumor cells stained with Aldefluor substrate and DEAB inhibitor. The Aldefluor substrate only reacts with the mammalian ALDH enzyme, so inclusion of the substrate will allow the human cells to shift forward while the chick cells will show no shift from the unstained sample. c Removal of the DEAB inhibitor results in a right-shift of the ALDH+ population. 1.55 % of the total cell population is ALDH+, but only 18.97 % of the total cell population is human cells. Therefore, 8.17 % of the UM-SCC-1 cells in the CAM xenograft were analyzed to be ALDH+

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Journal of Otolaryngology - Head & Neck Surgery

ISSN: 1916-0216

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