Fig. 3

Expression profiles of representative GR isoforms among subgroups representing different endotypes of CRSwNP and the control group. The relative expression levels of a the sum of bands 1–5 and representative bands for GRs, including b GRα-A/B, c GRα-C, d the 60 kDa protein and e GRα-D, which were detected with an anti-total GR antibody (PA1-511A), were analyzed among the E-CRSwNP subgroup, the NE-CRSwNP subgroup and the control group. Similar band intensity comparisons were performed to analyze f the sum of bands 1–5 and the bands for g GRα-A/B, (H) the 67 kDa protein, and i GRα-D, which were detected with an anti-GRα antibody (PA1-516). Data was presented as a ratio of each isoform to an internal control which were relative to β-actin expression. The data were presented in dot plots with medians and interquartile ranges. GR, glucocorticoid receptor; CRSwNP, chronic rhinosinusitis with nasal polyps; kDa, kilodaltons; E-NP, eosinophilic nasal polyp; NE-NP, noneosinophilic nasal polyp