Fig. 3

A Assessment of ZNF154 and ZNF132 transcripts in HEK-293 and UM-SCC-1 transductant cells and empty vector controls by real-time PCR. RNA transcript measurements are expressed as ΔΔCt values using GAPDH as a measurement control. B Western blot assessment of the same lentiviral transductants overexpressing ZNF154 or ZNF132 as probed using a Flag antibody. Also shown are empty vector controls for HEK-293 (H) and UM-SCC-1 (U). β-actin is used as a loading control. C PCR amplification of integrated vector DNA constructs for ZNF154-Flag, ZNF132-Flag, and empty vector control in both HEK-293 (H) and UM-SCC-1 cells (U). Stock vector DNA and a water blank were used as PCR positive and negative controls, respectively. D Schematic diagram showing locations of deleted internal coding sequences for each ZNF gene in UM-SCC-1 transductants as determined by sequencing of genomic DNA PCR products