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Fig. 4 | Journal of Otolaryngology - Head & Neck Surgery

Fig. 4

From: Effects of early local administration of high-dose bFGF on a recurrent laryngeal nerve injury model

Fig. 4

A Representative microscopy images of double staining with myogenic differentiation (MyoD) antibody and 4ʹ,6-diamidino-2-phenylindole dihydrochloride (DAPI) on day 14 after high dose basic fibroblast growth factor (bFGF) injection in the paralyzed side, or saline injection in the paralyzed side or non-paralyzed side (400 × magnification). The number of MyoD+ myoblasts in the paralyzed side of the high-dose group was higher than that in the non-paralyzed and paralyzed side of the saline group. White arrows indicate MyoD+ cells. B Temporal changes in the ratio of MyoD+ myoblasts to muscle fibers after bFGF injection in the paralyzed side or saline injection in the paralyzed or non-paralyzed side. Error bars represent standard deviation. **P < .01, Tukey’s multiple comparisons tests. Saline group NPS, non-paralyzed side of the saline group; Saline group PS, paralyzed side of the saline group; High-dose group, paralyzed side of the high-dose basic fibroblast growth factor group

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