Correlation of PET-CT nodal SUVmax with p16 positivity in oropharyngeal squamous cell carcinoma
© Clark et al. 2015
Received: 4 June 2015
Accepted: 2 September 2015
Published: 15 September 2015
The incidence of human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (OPSCC) has been rising in recent years. Given the clinical impact of HPV/p16 positivity in OPSCC, identifying surrogate markers of this disease early in the diagnostic work-up of these patients could improve patient care.
Demographic, pathologic, staging and PET-CT data from patients diagnosed with OPSCC from 2009–2014 were obtained from a prospectively collected provincial cancer registry. Tumor HPV/p16 status was correlated to the maximum standard uptake value (SUVmax) of the primary tumor and cervical nodes. Comparisons of means and multinomial regression models were used to determine associations between p16 status and SUVmax. A diagnostic odds ratio was calculated using a cut off value for predicting HPV/p16 positivity based on nodal SUVmax.
PET-CT and HPV/p16 data was obtained for 65 patients treated surgically for OPSCC. Significantly higher nodal SUVmax was associated with HPV/p16 positive nodes (SUVmax 10.8 vs 7.9). No significant differences were seen between HPV/p16 positive vs negative primary tumor SUVmax (10.3 vs 13.7). In combination with other clinical parameters, higher nodal SUVmax was highly correlated with HPV/p16 positivity.
Elevated nodal SUVmax is a significant predictor of HPV/p16 positive disease.
KeywordsOropharyngeal cancer Positron emission tomography Standard uptake value p16 Human papillomavirus
Oropharyngeal squamous cell carcinoma (OPSCC) is an aggressive malignancy with a rising incidence worldwide [1–3]. Oncogenic human papillomavirus (HPV) infection, tobacco and alcohol are well established etiological factors for OPSCC [4, 5]. A large body of evidence from recent years has demonstrated that HPV positive and negative OPSCC are distinct from clinical, pathological and molecular perspectives [4, 6–12]. Most importantly; patients with HPV-related OPSCC, generally pathologically identified by p16 positivity, have favorable survival outcomes following both surgical and non-surgical treatment approaches [4, 5, 13]. As recommended by recent head and neck cancer treatment guidelines, it is important to establish a pathologic diagnosis of HPV/p16 OPSCC for purposes of prognostication . Despite well-characterized clinical and histopathological differences, the treatment algorithm for HPV/p16 positive and negative OPSCC remains unchanged.
Positron emission tomography-computed tomography (PET-CT) is an important tool for the diagnosis and surveillance of OPSCC [12, 15, 16]. PET-CT utilizes glucose metabolism to identify tumor metabolic activity, which can accurately identify locoregional disease better than CT alone, particularly in smaller nodes [17, 18]. Rates of cervical lymph node metastases in HPV positive OPSCC have been reported to be 60-76 % at initial presentation . Sensitivity and specificity of detecting cervical node metastases with PET-CT is high, ranging from 84-92 % and 95-99 %, respectively . HPV positive nodal metastases often present as a cystic or necrotic neck mass which may be less metabolically active on PET . Additionally, inflammatory nodes can present as FDG avid lymphadenopathy and could represent false positives on PET imaging. Combining PET with CT allows for precise anatomic localization and further characterization of these potential metastatic lymph nodes.
The clinical role of PET-CT to localize primary tumors and metastatic disease has been well-established; but researchers are continuing to try to identify specific imaging biomarkers that may help diagnose, provide information about prognosis and guide treatments for patients with OPSCC. Maximum standard uptake value (SUVmax) is a quantitative measure of the highest FDG uptake within the region of interest. It has been hypothesized by others that a high SUVmax would be associated with a poor outcome, reflecting the increased proliferation of the tumor cells . However HPV positive tumors, known to have favorable outcomes, are thought to have higher metabolic activity with increased proliferation and a predilection for early metastasis [22, 23]. Recent data from Kendi et al.  support the hypothesis that higher SUVmax is associated with HPV/p16 positivity. There is therefore conflicting evidence regarding the association between SUVmax and HPV/p16 positivity in OPSCC [21, 24–26]. To this end, we aimed to determine the correlation between nodal SUVmax and p16 status of OPSCC tumors using a cohort of patients treated with primary surgery.
The University of Alberta Research Ethics Board granted ethics approval for the study (Pro00016426).
PET-CT image analysis
PET-CT protocols were obtained using two scanners, both Gemini TF 16-slice PET-CT scanner systems (Phillips Healthcare, Andover, MA). All patients were scanned with a consistent head and neck protocol. Following a fasting period of at least 4 h and serum baseline glucose measurements, patients were injected with 5.18 MBq/kg of 18-FDG tracer. A minimum of 60 min was waited following tracer injection and PET scanning. Two separate acquisitions were used to scan patients from skull vertex to mid thighs. Following the PET scan, patients were injected with intravenous contrast and a helical CT was performed to scan patients from skull vertex to mid thighs.
Using OASIS software (Segami Corporation, Columbus, Ohio), PET-CT images were reviewed by authors JC, CJ and TC. Reviewers were blinded to p16 status at the time of imaging review. Coronal, sagittal and transverse images were examined to identify SUVmax of the primary tumor and cervical nodal metastases. A spherical volumetric area of interest was focused upon individual lesions in the head and neck to identify metabolic parameters of the lesions. Final radiological and nuclear medicine reports were reviewed for additional correlation of findings. Only nodal and primary malignancies with FDG uptake were included within the analysis. The SUVmax for the primary as well as the nodal SUVmax were obtained from the OASIS software. In patients with multiple FDG avid cervical lymph nodes, the highest SUVmax was recorded, consistent with previous PET-CT protocols .
SPSS version 23.0 was used for statistical analyses (SPSS Inc., Chicago, IL, USA). Regression analyses of factors and covariates were performed to include age, gender, TNM staging, tumor subsite, treatment, smoking status, p16 positivity and SUVmax. ANOVA, Mann Whittney U test, and the Kruskal-Wallis test were used to calculate differences between groups where appropriate. Statistical significance was defined as p < 0.05.
A prospectively collected database identified 167 patients who were treated at the University of Alberta for OPSCC between 2009 and 2014. Of the patients treated with primary surgery, sixty-five had adequate pre-treatment PET-CT and p16 status available (Fig. 1).
Demographics and staging of oropharyngeal malignancies
Age (mean, SD)
Gender (% male)
Multinomial analysis with p16 status as the dependent variable
Primary SUV max
Using the mean SUVmax of p16 positive nodes as a significant predictor of p16 positive disease, we categorized patients according to this cutoff value (mean SUVmax p16+). Patients with nodal SUVmax ≥ mean SUVmax p16+ had a positive predictive value of p16 positive disease of 92.3 %. The diagnostic odds ratio (DOR) of the mean SUVmax p16+ was 4.9 (95 % CI, 1.0-24.3)
The emergence of PET-CT as a diagnostic tool for head and neck malignancies has allowed physicians to identify primary tumors and locoregional metastases early in the disease course. Many feel the role of PET-CT can be expanded to help characterize some of these lesions to more accurately diagnose and target therapy for different types of cancers. This study has shown a significant association between higher nodal SUVmax and p16 positive disease status. In addition, in patients who had a nodal SUVmax of greater than 10.8 (mean SUVmax p16+), we could reliably predict p16 positive disease (DOR = 4.9). A biological basis for these findings could represent the higher cell division and oxygenation of p16 positive tumors or the immune response of HPV infected nodes, which could FDG activity .
Two similar studies examining the association of PET-CT and HPV status have found conflicting results [24, 25]. Tahari and colleagues had a large cohort (N = 123) and retrospectively examined the relationship between PET-CT imaging markers and HPV status . They found higher PET values in HPV positive nodal metastasis but this difference was not statistically significant. Kendi and colleagues  examined the relationship of PET-CT imaging markers and p16 status in patients with oral cavity and OPSCC . Consistent with results from our study, they found a statistically significantly higher nodal SUVmax, but not primary tumor SUVmax, in patients with HPV positive disease as compared to those with HPV negative cancer. Although their study had a relatively small sample size (N = 22), they also identified a nodal SUVmax cut off value, which was predictive of HPV positivity (SUVmax >7.66). Our study supports a significant association between p16 status and nodal SUVmax. With this data, clinicians may be able to more accurately predict HPV status based on imaging in combination with other clinical parameters.
HPV positive tumors are being found in a new population – younger males without environmental risk factors such as tobacco and alcohol. Clinicians; however, may be slow to screen these individuals for malignancies, as they do not have the traditional exposures that have been historically associated with head and neck cancer. As a result, the average time for diagnosis of OPSCC is greater than 3 months from initial presentation at a general practioner’s office . Given the prognostic importance of p16 positivity in OPSCC, diagnostic information that can assist in triaging patients according to p16 status is valuable to both patients and physicians.
PET-CT also plays a role in identifying primary tumors in cases of cancer of unknown primary (CUP). Multiple studies have found HPV positive metastatic neck nodes are more likely to be associated with an oropharyngeal primary than other head and neck malignancies [31, 32]. Nodal SUVmax, identified on pre-treatment PET-CT could give additional information about the underlying malignancy for CUP. Having a high suspicion for p16 positive OPSCC based on imaging could help direct a more aggressive search for a CUP. Recent reports using transoral resection of base of tongue and tonsils in p16 positive CUPs have been >90 % effective in identifying the primary site [33, 34].
Our findings could have a particularly useful clinical implication in the diagnosis of OPSCC patients when surgical biopsy under general anesthesia may portend a high risk of morbidity. If imaging with PET-CT could help identify p16 status, clinicians may be able to tailor chemoradiation therapy in this population. It is thought the improved prognosis of HPV positive OPSCC relates in part to the increased radiosensitivity of the tumor. Currently HPV positive and negative tumors are treated equivocally. Given the variable pathologic and molecular behavior of HPV positive and negative tumors, targeted treatments may soon be developed that could allow for individualized therapies that may reduce overall radiation-related side effects and improve post-treatment quality of life.
This study has a number of limitations. Although initial data were collected prospectively, it remains a retrospective analysis in a single institution, which could limit the generalizability of the results. As shown in Fig. 1, several patients were excluded from the analysis due to lack of PET-CT or p16 status, which could bias our results. PET-CT protocols may vary between cancer treatment facilities, which may limit the extrapolation of nodal SUVmax cutoff values described herein. Finally, a relatively small patient cohort was used in this analysis. These limitations could be improved with further studies using prospective data from multiple institutions.
There is a significant association between p16 positivity and increased nodal SUVmax in OPSCC. Further larger scale studies involving other institutions would be recommended to examine the clinical utility of this association.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Hocking JS, Stein A, Conway EL, Regan D, Grulich A, Law M, et al. Head and neck cancer in Australia between 1982 and 2005 show increasing incidence of potentially HPV-associated oropharyngeal cancers. Br J Cancer. 2011;104:886–91.PubMed CentralView ArticlePubMedGoogle Scholar
- Marur S, D’Souza G, Westra WH, Forastiere AA. HPV-associated head and neck cancer: a virus-related cancer epidemic. Lancet Oncol. 2010;11:781–9.View ArticlePubMedGoogle Scholar
- Westra WH. The changing face of head and neck cancer in the 21st century: the impact of HPV on the epidemiology and pathology of oral cancer. Head Neck Pathol. 2009;3:78–81.PubMed CentralView ArticlePubMedGoogle Scholar
- Ang KK, Harris J, Wheeler R, Weber R, Rosenthal DI, Nguyen-Tân PF, et al. Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med. 2010;363:24–35.PubMed CentralView ArticlePubMedGoogle Scholar
- Gillison ML, Zhang Q, Jordan R, Xiao W, Westra WH, Trotti A, et al. Tobacco smoking and increased risk of death and progression for patients with p16-positive and p16-negative oropharyngeal cancer. J Clin Oncol. 2012;30:2102–11.PubMed CentralView ArticlePubMedGoogle Scholar
- Andrews E, Seaman WT, Webster-Cyriaque J. Oropharyngeal carcinoma in non-smokers and non-drinkers: a role for HPV. Oral Oncol. 2009;45:486–91.View ArticlePubMedGoogle Scholar
- D’Souza G, Kreimer AR, Viscidi R, Pawlita M, Fakhry C, Koch WM, et al. Case–control study of human papillomavirus and oropharyngeal cancer. N Engl J Med. 2007;356:1944–56.View ArticlePubMedGoogle Scholar
- Kumar B, Cordell KG, Lee JS, Worden FP, Prince ME, Tran HH, et al. EGFR, p16, HPV Titer, Bcl-xL and p53, sex, and smoking as indicators of response to therapy and survival in oropharyngeal cancer. J Clin Oncol. 2008;26:3128–37.PubMed CentralView ArticlePubMedGoogle Scholar
- Biron VLV, Mohamed AA, Hendzel MJM, Underhill DDA, Seikaly HH. Epigenetic differences between human papillomavirus-positive and -negative oropharyngeal squamous cell carcinomas. J Otolaryngol Head Neck Surg. 2012;41 Suppl 1:S65–70.PubMedGoogle Scholar
- van Kempen PM, Noorlag R, Braunius WW, Stegeman I, Willems SM, Grolman W. Differences in methylation profiles between HPV-positive and HPV-negative oropharynx squamous cell carcinoma: A systematic review. Epigenetics. 2014;9(2):194-203.Google Scholar
- Cooper T, Biron V, Adam B, Klimowicz AC, Puttagunta L, Seikaly H. Prognostic utility of basaloid differentiation in oropharyngeal cancer. J Otolaryngol Head Neck Surg. 2013;42:57.PubMed CentralView ArticlePubMedGoogle Scholar
- Xu CC, Biron VL, Puttagunta L, Seikaly H. HPV Status and second primary tumours in Oropharyngeal Squamous Cell Carcinoma. J Otolaryngol Head Neck Surg. 2013;42:36.PubMed CentralView ArticlePubMedGoogle Scholar
- Seikaly H, Biron VL, Zhang H, O’Connell DA, Côté DWJ, Ansari K et al. The role of primary surgery in the treatment of advanced oropharyngeal cancer. Head Neck 2015. doi:https://doi.org/10.1002/hed.24042.
- Pfister DG, Ang K-K, Brizel DM, Burtness BA, Busse PM, Caudell JJ, et al. Head and neck cancers, version 2.2013. Featured updates to the NCCN guidelines. J Natl Compr Canc Netw. 2013;11:917–23.PubMedGoogle Scholar
- Pentenero M, Cistaro A, Brusa M, Ferraris MM, Pezzuto C, Carnino R, et al. Accuracy of 18 F-FDG-PET/CT for staging of oral squamous cell carcinoma. Head Neck. 2008;30:1488–96.View ArticlePubMedGoogle Scholar
- Kurien G, Hu J, Harris J, Seikaly H. Cost-effectiveness of positron emission tomography/computed tomography in the management of advanced head and neck cancer. J Otolaryngol Head Neck Surg. 2011;40:468–72.PubMedGoogle Scholar
- Muylle K, Castaigne C, Flamen P. 18F-fluoro-2-deoxy-D-glucose positron emission tomographic imaging: recent developments in head and neck cancer. Curr Opin Oncol. 2005;17:249–53.View ArticlePubMedGoogle Scholar
- Yoon DY, Hwang HS, Chang SK, Rho Y-S, Ahn HY, Kim JH, et al. CT, MR, US,18F-FDG PET/CT, and their combined use for the assessment of cervical lymph node metastases in squamous cell carcinoma of the head and neck. Eur Radiol. 2009;19:634–42.View ArticlePubMedGoogle Scholar
- Lin DT, Cohen SM, Coppit GL, Burkey BB. Squamous cell carcinoma of the oropharynx and hypopharynx. Otolaryngol Clin North Am. 2005;38:59–74. viii.View ArticlePubMedGoogle Scholar
- Corey AS, Hudgins PA. Radiographic imaging of human papillomavirus related carcinomas of the oropharynx. Head Neck Pathol. 2012;6 Suppl 1:S25–40.View ArticlePubMedGoogle Scholar
- Hoshikawa H, Yamamoto Y, Mori T, Kishino T, Fukumura T, Samukawa Y, et al. Predictive value of SUV-based parameters derived from pre-treatment (18)F-FLT PET/CT for short-term outcome with head and neck cancers. Ann Nucl Med. 2014;28:1020–6.View ArticlePubMedGoogle Scholar
- Arenz A, Ziemann F, Mayer C, Wittig A, Dreffke K, Preising S, et al. Increased radiosensitivity of HPV-positive head and neck cancer cell lines due to cell cycle dysregulation and induction of apoptosis. Strahlenther Onkol. 2014;190:839–46.View ArticlePubMedGoogle Scholar
- Ziemann F, Arenz A, Preising S, Wittekindt C, Klussmann JP, Engenhart-Cabillic R, et al. Increased sensitivity of HPV-positive head and neck cancer cell lines to x-irradiation ± Cisplatin due to decreased expression of E6 and E7 oncoproteins and enhanced apoptosis. Am J Cancer Res. 2015;5:1017–31.PubMed CentralPubMedGoogle Scholar
- Kendi ATK, Magliocca K, Corey A, Nickleach DC, Galt J, Higgins K, et al. Do 18F-FDG PET/CT parameters in oropharyngeal and oral cavity squamous cell carcinomas indicate HPV status? Clin Nucl Med. 2015;40:e196–200.View ArticlePubMedGoogle Scholar
- Tahari AK, Alluri KC, Quon H, Koch W, Wahl RL, Subramaniam RM. FDG PET/CT imaging of oropharyngeal squamous cell carcinoma: characteristics of human papillomavirus-positive and -negative tumors. Clin Nucl Med. 2014;39:225–31.PubMed CentralView ArticlePubMedGoogle Scholar
- Joo Y-H, Yoo I-R, Cho K-J, Park J-O, Nam I-C, Kim M-S. Preoperative 18F-FDG PET/CT and high-risk HPV in patients with oropharyngeal squamous cell carcinoma. Head Neck. 2014;36:323–7.View ArticlePubMedGoogle Scholar
- O Connell D, Seikaly H, Murphy R, Fung C, Cooper T, Knox A, et al. Primary surgery versus chemoradiotherapy for advanced oropharyngeal cancers: a longitudinal population study. J Otolaryngol Head Neck Surg. 2013;42:31.View ArticlePubMedGoogle Scholar
- Lau HY, Brar S, Klimowicz AC, Petrillo SK, Hao D, Brockton NT, et al. Prognostic significance of p16 in locally advanced squamous cell carcinoma of the head and neck treated with concurrent cisplatin and radiotherapy. Head Neck. 2011;33:251–6.View ArticlePubMedGoogle Scholar
- Begum S, Gillison ML, Ansari-Lari MA, Shah K, Westra WH. Detection of human papillomavirus in cervical lymph nodes: a highly effective strategy for localizing site of tumor origin. Clin Cancer Res. 2003;9:6469–75.PubMedGoogle Scholar
- Ho T, Zahurak M, Koch WM. Prognostic significance of presentation-to-diagnosis interval in patients with oropharyngeal carcinoma. Arch Otolaryngol Head Neck Surg. 2004;130:45–51.View ArticlePubMedGoogle Scholar
- Fakhry C, Westra WH, Li S, Cmelak A, Ridge JA, Pinto H, et al. Improved survival of patients with human papillomavirus-positive head and neck squamous cell carcinoma in a prospective clinical trial. J Natl Cancer Inst. 2008;100:261–9.View ArticlePubMedGoogle Scholar
- Schmalbach CE, Miller FR. Occult primary head and neck carcinoma. Curr Oncol Rep. 2007;9:139–46.View ArticlePubMedGoogle Scholar
- Mehta V, Johnson P, Tassler A, Kim S, Ferris RL, Nance M, et al. A new paradigm for the diagnosis and management of unknown primary tumors of the head and neck: a role for transoral robotic surgery. Laryngoscope. 2013;123:146–51.View ArticlePubMedGoogle Scholar
- Karni RJ, Rich JT, Sinha P, Haughey BH. Transoral laser microsurgery: a new approach for unknown primaries of the head and neck. Laryngoscope. 2011;121:1194–201.View ArticlePubMedGoogle Scholar